badionotus, and on most other types of sea cucumbers, browse in these rooked variety in Colombia might have been limited to a few knowledge towards variety and shipments [2, 16] and you may reproductive biology [17, 18]. not, there are still of a lot gaps inside the training, such as for example regarding their reproductive models and just how he’s determined by parameters such temperatures, salinity, water, and dinner supply.
Today’s study means aspects of the latest reproductive biology of the holothuroids We. badionotus and you can Isostichopus sp. regarding the Santa Marta Area using the gonadal list (GI) and you can histological examination of the brand new gonads. This study is intended to increase the most recent knowledge about the fresh biology and you can ecology out of sea cucumbers native to the new Colombian Caribbean Ocean and gives a technical cause for the introduction of management measures and conservation for the investment.
dos. Materials and techniques
Specimens from Isostichopus sp. have been collected away from , if you find yourself specimens from Isostichopus badionotus had been built-up regarding in the Taganga Bay (11° 16′ ” N– 74° 11′ ” W) and you will Rodadero Bay (11° 12′ ” N– 74° 13′ ” W) regarding Caribbean region of Santa Marta, Colombia (Fig step one).
Additionally assesses the latest dictate out of environment and chemical compounds variables (temperature, rain, brand new portion of normal carbon dioxide posts in the sediment) to their reproductive biology and analyzes the dwelling of its communities (volume away from size and you can lbs, intercourse ratio, suggest size and you will pounds at first intimate maturity, fecundity)
Box in the inset A are magnified in the inset B; field inside the inset B are magnified in the main panel. Source: Guidance foot INVEMAR.
As a result of the wide variety of shapes and colors that i. badionotus has, this research focused on the fresh collection of organisms with well-known morphotype regarding the kinds found in the analysis urban area, described from the Vergara mais aussi al. when i. badionotus morphotype II.
Monthly fourteen to help you 20 folks of per morphotype (I. badionotus and you may Isostichopus sp.) had been accumulated because of the snorkeling. For this reason, a pair of snorkelers swam an effective transect 5 meters wider by the whenever a thousand m a lot of time, double. The original excursion is made bordering the brand new coastline line plus the second at a distance around 10 meters about coast range. Maximum search breadth try ten meters to own logistical and you can coverage factors and though it’s likely that you will find anybody on greater distance and you may breadth, this may not be reviewed in today’s research. When you look at the October, zero collections would be made because of the passage through of Hurricane Matthew, which affected the fresh Colombian Caribbean through that day. Most of the sea cucumbers have been directly packed in the synthetic handbags having seawater and you can transported on the Aquaculture Lab of Universidad del Magdalena. The fresh depth out of simply take of every private as well as the types of bottom it occupied was indeed registered (classified just like the rocky, exotic, coral, rock-coral, rock-mud, rock-sand-coral). While doing so, surface deposit examples (which have a thickness perhaps not more than ten mm) had been taken around the men and women to influence new portion of natural carbon dioxide posts using a bulk spectrometer, Euro EA 3000 elemental analyzer (EuroVector). Salinity and you will h2o temperature was basically registered each month, whenever single men dating in Los Angeles you are complete rain investigation had been obtained from the new information of your Simon Bolivar airport meteorological station of the town of Santa Marta.
Individuals was in fact forfeited on the research by setting them during the seawater at 4°C, which was and intended to come to a maximum county of contraction you to permitted to slow down the mistake while in the length specifications, due to the capabilities that they must increase otherwise deal from the tend to. Then your following looks proportions was taken: full looks size (TL), playing with a flexible 1-meters tape level during the nearby 0.5 cm (after compromise by thermal amaze the fresh developed body had a c otherwise S contour, therefore the tape contoured the human body, therefore the overall size ‘s the contour duration); gonad regularity having fun with a graduated tube in the nearest 0.1 cm step three ; system wall structure pounds (BW), identified as the extra weight of the individual in place of viscera or gonad and you may gonad weight (GW) in the nearby 0.step one g. Gonad index (GI) are computed using the formula, GI = (GW/ BW)* 100 [19, 20].
